Quantitative PCR
Quantitative PCR or real-time PCR (SYBR or TaqMan based) are used for a wide range of applications that require accurate quantification of target RNA or DNA. qPCR provides superior sensitivity, dynamic range, and precision, while eliminating the need for post-PCR sample processing.
Genotypic provides high-precision qPCR services using two leading platforms:
- Stratagene Mx3000P qPCR machine
- Roche Light Cycler 480 qPCR machine
Key Features:
Support for experimental design:
Tailored guidance on experimental setup to achieve optimal results.
Customized panel design:
Design of primers/probes based on TaqMan or SYBR chemistry for specific needs.
Preparation of high-quality DNA/RNA samples:
Ensuring the integrity of your samples for reliable qPCR results.
High-performance assays:
Designed and executed by an experienced team of professionals to ensure superior data quality.
Applications:
Gene Expression Profiling:
Study gene regulation and modulations in expression, including epigenetic factors.
Empirical Validation:
Validate NGS and Microarray data for better accuracy and reliability.
miRNA Expression Profiling:
Detect changes in gene expression in response to diseases or drug therapies.
Absolute Quantification:
Precisely measure the copy number of target nucleic acid molecules.
Pathogen Detection:
Identify and quantify pathogens with high sensitivity.
Viral Quantification:
Quantify viral loads in biological samples.
Genetic Variation Analysis:
Perform genotyping and detect mutations in genes of interest.