Molecular Biology Services

Nucleic acid extraction services

Reliable genomics data is derived from samples with high quality nucleic acids, be it RNA or DNA. Genotypic offers nucleic acid isolation services for all types of DNA and RNA from a variety of organisms and tissue types for both Microarray and NGS applications. Genotypic has experience of extracting RNA and DNA from several hundred different organisms and tissue types including prokaryotes, viral samples, lower eukaryotes, plants and animal species and metagenomes from a variety of samples like soil, water, sludge, fecal matter etc. We also offer mitochondrial DNA, chloroplast DNA and serum nucleic acids isolation services.

Integrity and purity of isolated nucleic acids are checked using Agilent 2100 Bioanalyzer and Agilent Tape station and quantified using Nanodrop and Qubit fluorometer.

Key Features:

From project planning to publication quality report

Consultation on tissue types, modes of harvesting, storage and transport

Fully equipped laboratory facilities with highly qualified professionals

Strict QC ensuring appropriate concentration/yield and integrity

qPCR services:

Quantitative PCR or real-time PCR (SYBR or TaqMan based) are used for a wide range of applications that require accurate quantification of target RNA or DNA. qPCR provides superior sensitivity, dynamic range, and precision, while eliminating the need for post-PCR sample processing.

Key Features:

Support for experimental design

Preparation of high-quality DNA/RNA samples

Design of customized primers/Probe based on TaqMan or SYBR chemistry.

High performance assays designed and executed by experienced team of professionals

Genotypic provides qPCR services on two platforms:

  • Stratagene Mx3000P qPCR machine
  • Roche Light Cycler 480 qPCR machine

Applications:

Gene expression profiling analysis to study epigenetic gene regulation and to quantify and analyse modulations in gene expression.Empirical Validation of NGS and Microarray data

miRNA Expression Profiling Analysis to detect changes in disease or in response to drug therapies

Absolute quantification to determine precise Copy Number of a target nucleic acid molecule within a sample

Pathogen Detection

Viral Quantification

Genetic variation analysis by genotyping and detection of mutations

Please write to us at genomics@genotypic.co.in to get more information.

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