About Next Generation Sequencing (NGS)
More than 100 times economical than Sanger sequencing!
Extremely High throughput- Sequence 100s of bacterial, small organism genomes in a day
Sequence complete human genome in a week
DNA and RNA Sequencing of organisms with little or no reference information available
Advanced Bioinformatics tools to support NGS data output now available - De-novo assembly, Contig validations and Mutation discovery
Data from NGS studies can be used to custom design unique microarrays
100s of publications using NGS since 2007
NGS applications
Whole genome sequencing
Targeted Re-sequencing
SNP and Mutation identification
Transcriptome sequencing
Digital gene expression
ChIP sequencing
Methylome sequencing
Small RNA sequencing
Targeted Re-sequencing @ Genotypic
Full service available for all organisms
Sequence selected genes / genomic regions in multiple samples combined with DNA Capture using Agilent SureSelect Technology - On-Array DNA Capture and In-Solution (Liquid) Capture
Sample/ Library preparation, Multiplexing options
Compatible with all NGS platforms
Popular NGS Themes
Bacterial and other microbial sequencing- De-novo and Re-sequencing of multiple strains, phenotype specific mutations, Vaccine development
Human Genetics- Detection of Known and New Mutation in specific genes, regions in large (hundreds) number of disease and control samples.
Disease Inheritance studies- Mutation Detection in Inherited Clinical Disorders- Deep sequencing of selected regions in family members with affected individual(s)
Transcriptome/RNA sequencing of organism with limited reference information available and then design custom microarray
Metagenomics- Intestinal microbial flora, Soil, marine Ecosystems and other microenvironments
Agrigenomics-: species of commercial/ research interest- Transcriptome, Whole genome, targeted resequencing of QTL loci
Pathogen detection in host system- foreign DNA sequence detection and characterization in clinical samples, plant and animal infections
ChIP Sequencing- Alternate to using ChIP on chip microarrays. Discover un-identified protein binding regions
Please fill following form or write to us at
genomics@genotypic.co.in to discuss your
requirement and avail special offers
DeNovo Sequencing
Please fill following form or write to us at
genomics@genotypic.co.in to discuss your requirement and avail special offers
Re-Sequencing
Click
here to download Genotypic On Array Capture work flow
Sequence Data Analysis
Data analysis for all Next Generation Sequencing platforms (Illumina, 454 & SOLiD)
Analysis performed for
a) Re-sequencing
i. QC of Next Generation Sequencing data
- QC of sequenced data using In-house techniques, keeping quality measure in mind
- QC of the Reference sequence for re-sequencing project
- Alignment statistics to find goodness of alignment for re-sequencing projects.
- Multi-level QC work-flows to ensure minimum error rate in analysis.
ii. Variance analysis
- SNP calling on reference sequence alignment
- Manual verification of mutations
- Annotation for genes affected by the mutations
- Mapping mutations on the genome
- Advanced Bioinformatics to determine biological importance of mutations in the coding region
iii. ChIP-Seq analysis
- Peak detection for enriched regions
- Create visualization of peaks
- Finding genes related to Enriched region
- Motif mapping and novel motif discovery
iv. Enrichment analysis for genome partition/focused re-sequencing
- Analysis using Agilent supported tool for genome focus captures sequencing
- Enrichment summary statistics
- Finding genes related to Enriched region
- Standard genome browser loadable files (UCSC Wiggle file and Bed file) to view the enriched regions
b) De-novo sequencing
i. Assembly Statistics
High throughput data analysis
Access to worlds 4th fastest super computer (EKA) outside government facilities through Genotypic's collaboration with CRL Pune.
Validation
Click
here to view Pre & Post sequencing applications using Microarray
