Third generation sequencers are being used worldwide to rapidly identify strains for epidemiological study of epidemics like EBOLA and currently for Coronavirus. Genotypic, the exclusive distributor for Nanopore Products in India supplies the sequencers and validated reagents for rapid testing & sequencing of SARS-CoV-2.

Why Nanopore Sequencers:

1. On-field portable sequencer
2. Low cost, affordable sequencer with minimal lab infrastructure
3. Quick turnaround time
4. Real time data generation & visualization
5. Optimized analysis pipeline for consensus generation and SNP analysis

We provide complete solution for setting up genomics lab for SARS-CoV-2 sequencing & testing:

Instruments:

• MinION
• Flowcells
• Control System
• Qubit Fluorometer
• Magnetic Stand

Reagents for Sample processing:

• DNA Polymerase
• Random hexamer Mix
• 10 mM dNTP solution
• Reverse transcriptase
• 40 U/μl SARS-CoV-2 primer (Pool A & B) 100uM (For ARTIC protocol)

Library preparation:

• End Repair/dA-Tailing Module
• Ligation Module
• Native Barcoding kit (1-12)
• DNA Repair Mix
• Ligation Sequencing Kit (1D)
• SpotON Flow cell R9.4
• AMPure XP - 60 ML
• Native Barcoding kit (13-24)
• Quant-iT dsDNA HS Assay Kit (500 assays, 2-1000 ng)

Control system & Software:

• MinKnow
• Guppy
• Commander with COVID analysis pipeline

Why ARTIC Protocol: Highly efficient amplification with ARTIC primer pools to achieve uniform amplification of complete genome.

Download COVID Sequencing Flyer
Download COVID Reagents Flyer

For further queries or collaborations contact us or write to us at nanopore@genotypic.co.in

Experience the power of the rapid sequencing of SARS-CoV-2 genome with our Nanopore Sequencing services! Viral Genome data is essential for drug and vaccine development and epidemiology. It also helps identify mutations which could influence the severity of the disease.

SARS-CoV-2 Sequencing Services include:

• Epitranscriptomics
• Whole Genome Sequencing
• Metagenomics
• Host response analysis through transcriptomics
• Any other customized services

Why Genotypic services for SARS-CoV-2 sequencing:

• Experienced team handling Nanopore sequencers for more than 3 years and successfully completed over 300 projects
• Quick Turn around Time
• One stop solution for your sequencing and data analysis requirements
• We use globally validated protocols to achieve uniform amplification of complete genome, consensus generation and reliable SNP analysis

Download COVID services at Genotypic Flyer

To send your samples for sequencing contact us and our executive will get in touch with you regarding the same.
Please note samples sent for sequencing should be sent as cDNA and we do not accept any clinical samples.

Nanopore Covidseq training:
Our experienced professionals will train you to establish SARS-CoV-2 sequencing right from setting up the sequencer to performing sequencing run. This training can be customized to be conducted onsite or online.
Details of training as follows:

1. Introduction to nanopore sequencing and guidance on performing Flow cell QC.
2. Training on RNA to cDNA conversion.
3. Training on PCR amplification of covid genome using overlapping primers.
4. Training on Nanopore library preparation and QC as per Artic protocol by using Native barcoding kit (EXP-NBD104) and Ligation sequencing kit (SQK-LSK109).
5. Training on flow cell loading and initiation of sequencing run.
6. Training on completing the sequencing run, data conversion (Basecalling) and Demultiplexing.

Nanopore COVID analysis training:
We also give customized data analysis training for COVID samples. The Sequence analysis training according to ARTIC pipeline using Commander Analysis software includes:

1. Base calling & barcode separation
2. Adapter trimming
3. Alignment
4. Variant calling
5. Consensus generation

To know more details about the training & get quotation please contact us.

1. Three adjacent nucleotide changes spanning two residues in SARS-CoV-2 nucleoprotein: possible homologous recombination from the transcription-regulating sequence
2. Characterisation of the transcriptome and proteome of SARS-CoV-2 using direct RNA sequencing and tandem mass spectrometry reveals evidence for a cell passage induced in-frame deletion in the spike glycoprotein that removes the furin-like cleavage site
3. Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study
4. The architecture of SARS-CoV-2 transcriptome
5. Amplicon based MinION sequencing of SARS-CoV-2 and metagenomic characterisation of nasopharyngeal swabs from patients with COVID-19