Nanopore Covidseq training:
Our experienced professionals will train you to establish SARS-CoV-2 sequencing right from setting up the sequencer to performing sequencing run. This training can be customized to be conducted onsite or online.
Details of training as follows:
- Introduction to nanopore sequencing and guidance on performing Flow cell QC.
- Training on RNA to cDNA conversion.
- Training on PCR amplification of covid genome using overlapping primers.
- Training on Nanopore library preparation and QC as per Artic protocol by using Native barcoding kit (EXP-NBD104) and Ligation sequencing kit (SQK-LSK109).
- Training on flow cell loading and initiation of sequencing run.
- Training on completing the sequencing run, data conversion (Basecalling) and Demultiplexing.
Nanopore COVID analysis training:
We also give customized data analysis training for COVID samples. The Sequence analysis training according to ARTIC pipeline using Commander Analysis software includes:
- Base calling & barcode separation
- Adapter trimming
- Alignment
- Variant calling
- Consensus generation
To know more details about the training & get quotation please
contact us.